The Biuret test
Aim:
To test if a sample of food contains protein.
Equipment:
- Test tube
- Sodium Hydroxide
- Copper sulfate
- Food sample
Method:
1. Place about 2 mL of the sample into a test tube and add 5 drops of sodium hydroxide.
2. Add 5 drops of copper sulfate.
3. Shake the test tube gently from side to side.
Results that may happen:
If the solution remains blue, then no protein is present. If the solution changes to a violet colour then protein is present in the sample.
Conclusion/discussion/Results:
Our first experiment failed due to too much milk. Second try also failed, even we heated it. If it had any protein it should've changed colour from blue to violet. By adding sodium hydroxide and copper sulfate, the sample turned to blue colour. After shaking the sample it changes colour, it means it has protein.
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